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Bacteriophage degraKlebsiella K30 capsular polysaccharide . An NMR investigating of the 3,4-pyruvated galactose-containing double Structure and imm of an oligosaccharide repeating unit of the capsular polysaccharide of type III group B Streptococcus . A retool construction for the type III grouping B streptococcic polysaccharide antigen.We have derived oligosaccdes from the capsular polysaccharide of type III aggroup B Streptococcus by enzymatic hydrolysis of a specific backbone glycosidic bond utilizing an endo-beta-galactosidase from Flavobacterium keratolyticus . enzymatic digestion of the polyose produced oligosaccharide sherd of one or more pentasaccharide repeating units . On the base of 13C NMR , 1H NMR , and methylation psychoanalysis , it was established that the smallest digestion fragment was alpha-D-NeupNAc- ( 2 -- -- 3 ) -beta-D-Galp- ( 1 -- -- 4 ) - [ beta-D-Glcp- ( 1 -- -- 6 ) ] - beta-D-GlcpNAc- ( 1 -- -- 3 ) -beta-D-Gal .<br /><br />The isolation of this oligosaccharide is logical with the susceptibility of the beta-D-Galp- ( 1 -- -- 4 ) -beta-D-Glcp linkage in the backbone of the type III aggroup B strep polysaccharide and confirms that the polysaccharide is frame of a pentasaccharide iterate unit . High resolve 13C NMR spectroscopical studies point that , as in the case of the pentasaccharide , the terminal sialic acid rest of the type III aggroup B streptococcal polyose were linked to O-3 and not to O-6 of its separate beta-D-galactopyranosyl remainder as had been previously reported ( Jennings , H. J. , Rosell , K.-G. , and Kasper , D. L .<br /><br />( 1980 ) Can . J. [http://www.allinno.com/product/food/684.html Seebio Colanic acid polymer] , 112-120 ) . This linkage was confirmed in an autonomous methylation analysis of the type III radical B streptococcal polyose . Thin level chromatogram binding assay and radioactive antigen bandage assays with radiolabeled oligosaccharides demonstrate the I repeat unit pentasaccharide oligosaccharide to be peaked antigenic . Increasing oligosaccharide size to a decasaccharide consisting of two repeating units resulted in an 8-fold increase in antigen binding in the direct radioactive antigen tie attempt .<br /><br />The results suggest that a region of the immunodeterminant site vital for antibody constipate is site in the backbone of the polyose and involves the beta-D-galactopyranose- ( 1 -- -- 4 ) beta-D-glucopyranose bond.Degradation of polysas by oxidization and beta-elimination.A Novel Family of R protein Regulate Polysaccharide metabolism in J Bacteriol . 2021 O203 ( 21 ) : e0038321.Human gut microbiome composion is constantly changing , and diet is a Major driver of these changes . [https://en.wikipedia.org/wiki/Colanic_acid Seebio Colanic acid polymer] that prevail in mammal legion for long stop of time must possess mechanics for sensing and adapting to nutrient sack to avoid being outcompeted . globose regulative mechanics arbitrate by RNA-binding proteins ( RBPs ) that order reaction to nourishing fault have been characterise in Proteobacteria and Firmicutes but stay unexplored in the Bacteroidetes .<br /><br />Here , we report the designation of RBPs that are broadly distributed crossways the Bacteroidetes , with many genomes encryption multiple imitate . gene encoding these RBPs are highly expressed in many Bacteroides mintage . A purified RBP , RbpB , from Bacteroides thetaiotaomicron bandage to single-stranded RNA in vitro with an affinity exchangeable to other qualify regulative RBPs . B. thetaiotaomicron mutation lacking RBPs show striking teddy in formulation of polyose utilization and capsular polyose loci , suggesting that these RBPs may act as global regulators of polysaccharide metamorphosis . A B. thetaiotaomicron ΔrbpB mutant render a growth desert on dietetical saccharify belonging to the raffinose family of oligosaccharides ( RFOs ) .<br /><br />The ΔrbpB mutant had thin manifestation of BT1871 , encoding a anticipate RFO-degrading melibiase , compared to the wild-type strain . sport of BT1871 affirm that the enzyme it encodes is requisite for increase on melibiose and further ontogeny on the RFOs raffinose and stachyose . Our data reveal that RbpB is require for optimum expression of BT1871 and former polysaccharide-related genes , advise that we have place an crucial new family of global regulatory proteins in the Bacteroidetes . IMPORTANCE The man Colon household 100 of bacterial species , including many belonging to the genus Bacteroides , that aid in founder down our food to keep us goodly . Bacteroides have many cistron responsible for burst down unlike dietary sugar , and composite regulative mechanisms ascertain that specific factor are only expressed when the correct saccharide are usable .