Infections-Devices-Contact-Eye-z
For instance, 56% of corneal ulcers in the United States are associated with contact lens wear. Bacterial biofilms may participate in ocular infections by allowing bacteria to persist on abiotic surfaces that come in contact with, or are implanted in the eye, and by direct biofilm formation on the biotic surfaces of the eye. Bacterial biofilm formation of the role of bacterial biofilm formation in ocular infections may aid in the development of future antimicrobial strategies in ophthalmology. We review the current literature and concepts relating to biofilm Inhibitory effect of direct electric field and HA-ZnO composites on S. aureus In addressing the issue of prosthetic infection, we demonstrate herein how direct electric field (DC EF) stimulation can effectively inhibit biofilm formation, when pathogenic Staphylococcus aureus (MRSA, USA 300) are grown on preincubation for 4 h, a low intensity DC EF (1V/cm) was applied for different maturation were evaluated by a combination of biochemical assays, fluorescence/confocal microscopy, and flow cytometry. The results confirm a time-dependent and composition-independent decrease in bacterial viability and analysis indicated that 12 h EF application resulted in membrane depolarization of ∼35% of S.
aureus populations on HA-xZnO composites. The live/dead assay results revealed ∼60% decline in viable bacterial numbers with a concomitant 3-fold increase in the production of reactive oxygen species (ROS) after 18 h of EF. The loss in bacterial viability and biofilm instability is due to the synergistic bactericidal action of ZnO and EF. Taken together, the use of engineered biomaterial substrate with antimicrobial reinforcement coupled with continuous low intensity EF application can be adopted to treat prosthetic [Article in Chinese; Abstract available in Chinese from the publisher] It was known that bacteria adhere to surfaces and form sessile colonies called biofilms. Biofilms show potential applications for biodegradation and biocatalysis, whilst they also cause healthy and environmental problems. In particular, they lead to human infections and biofouling problems in industry. Physical properties of biofilms reflect the architecture and mechanical stability of biofilms that are highly related to their resistance to environmental challenges and their survival.
In this article, we reviewed the physical properties involved in the development of biofilms and the related characterization techniques. The surface adhesion of bacteria plays a crucial role in the biofilm formation, which is determined by the motion of bacteria near a surface as well as the interaction between the bacteria and the surface. As far as the biofilms become mature, they behave like a polymer glassy material 生物被膜涉及到人类生产生活的方方面面。生物被膜的形成有时是有益的，可用于生物降解、生物催化等；但同时也造成了诸多不利的影响，医疗领域中的感染性疾病、工业生产中的生物污损等均与生物被膜的形成有关。生物被膜形成过程中的物理性质决定着生物被膜的形态结构以及机械稳定性，对它在应对外界环境刺激并得以生存具有重要的意义。本文介绍了生物被膜形成初期和发展过程中的物理性质以及相应的表征手段。其中，细菌的表面粘附由细菌的近界面运动行为及细菌与表面的相互作用决定，并对生物被膜的初期形成起关键的作用。此外，机械性能测试发现成熟的生物被膜可看作具备粘弹性的聚合物。. Pattern formation exhibited by biofilm formation within microfluidic chambers. This article investigates the dynamics of an important bacterial pathogen, Xylella fastidiosa, within artificial plant xylem. The bacterium is the causative agent of a variety of diseases that strike fruit-bearing plants including Pierce's disease of grapevine. Biofilm colonization within microfluidic chambers was visualized in a laboratory setting, showing robust, regular spatial patterning.
We also develop a mathematical model, based on a multiphase approach that is able to capture the spacing of the pattern and points to the role of the exopolymeric substance as the main source of control of the pattern dynamics. We concentrate on estimating the attachment/detachment processes within the chamber because these are two mechanisms that have the potential to be engineered by applying various chemicals to prevent or treat the Anti-biofilm peptides as a new weapon in antimicrobial warfare. Microorganisms growing in a biofilm state are very resilient in the face of treatment by many antimicrobial agents. Biofilm infections are a significant problem in chronic and long-term infections, including those colonizing medical devices and implants. Colanic acid -biofilm peptides represent a very promising approach to treat biofilm-related infections and have an extraordinary ability to interfere with various stages of the biofilm growth mode.
