Carbohydrate-Content-Psa-Site-Protein-q
The proposed carbohydrate structure is a diantennary N-linked oligosaccharide of the N-acetyllactosamine type, with a sialic acid group at the end of each of the two branches. In addition, our data indicate that approximately % of the PSA molecules contain a fucose group in the core chitobiose moiety. The calculated molecular weight of this carbohydrate structure (M(r) 2,351) is in excellent agreement with the predicted molecular weight of the carbohydrate group, based on the M(r) 28,4 for PSA measured by ion spray mass spectrometry and M(r) 2679 calculated from the consensus sequence for the peptide portion of the molecule. ISMS of PSA is thus proposed as a convenient and reliable method of quality control, an indispensible step towards international standardization of this very important tumor marker for detection and monitoring of prostatic Quantitative chromatography of the oligosaccharides in wort, beer and brewing Nutritional aspects of short-chain fructooligosaccharides natural occurrence, chemistry, physiology and health implications.Short-chain fructooligosaccharides occur in a number of edible plants, such as chicory, onions, asparagus, wheat..
. They are a group of linear fructose oligomers with a degree of polymerisation ranging from n = 1 up to 5 escape digestion in the human upper intestine and reach the colon where they are totally fermented mostly to lactate, short chain fatty acids (acetate, propionate and butyrate), and gas, like dietary fibres. As a consequence of their fermentation, their caloric value is approximately 2 Kcalg. A faecal bulking effect of fructooligosaccharides has been observed in humans. An important property of short-chain fructooligosaccharides is the stimulation of bifidobacterial growth specifically while suppressing the growth of potentially harmful species such as, for example, Clostridium perfringens in the colon. It is associated with a decrease in faecal pH, an increase in faecal or colonic organic acids, a decrease in the production of nitrogenous end products in urine and stools, a decrease in faecal bacterial enzymatic activities and a modification in faecal neutral sterols. The short-chain fructooligosaccharides enhance magnesium absorption in humans and have been shown, in animal models, to reduce colon tumour development by enhancing both colon butyrate concentrations Effect of energy depletion on the glycosylation of a viral glycoprotein.
In lacto n neotetraose -depleted cells the formation of mannosylphosphoryldolichol is inhibited. Glucosyl-phosphoryldolichol, di-N-acetylchitobiosyl-pyrophosphoryl-dolichol and also GDP-mannose are still present, indicating that the formation of mannosyl-phosphoryl-dolichol and hence glycosylation of proteins via the lipid-linked oligosaccharide the energy-depleted cells the endo-beta-N-acetylglucosaminidase H-resistant, lipid-linked oligosaccharides (Man)x(GlcNAc)2-pyrophosphoryl-dolichol (X = 1 to 5) are still formed. Despite the absence of mannosyl-phosphoryl-dolichol protein glycosylation is not blocked. The influenza virus hemagglutinin is glycosylated with endo-beta-N-acetylglucosaminidase H-resistant, but not complex, oligosaccharides. These oligosaccharides can be processed to protein-linked However, the rate at which the hemagglutinin leaves the rough endoplasmic reticulum and, probably therefore, the rate of oligosaccharide-processing are decreased in the energy-depleted cells. Both inhibition of formation of mannosyl-phosphoryl-dolichol and of intracellular migration are reserved when the energy status is brought back to normal levels.Structure of oligosaccharides on Saccharomyces SUC2 invertase secreted by the Saccharomyces SUC2 invertase, secreted by the methylotrophic yeast Pichia pastoris and purified to homogeneity from the growth medium by DE-52 chromatography, appeared on sodium dodecyl sulfate-polyacrylamide gel electrophoresis as a diffuse ladder of species at 85- kDa, while the secreted Saccharomyces form migrated as a broad band from 0 to 1 kDa.
Endo-beta-N-acetylglucosaminidase H released the Pichia invertase carbohydrate generating a -kDa protein with residual Asn-linked GlcNAcs and oligosaccharides separated on Bio-Gel P-4 into Man8-11GlcNAc. Nearly 75% of the oligosaccharides were equally distributed between Man8,9GlcNAc, while 17% were ManGlcNAc and 8% were Man11GlcNAc. Oligosaccharide pools were analyzed for homogeneity by high-pH anion-exchange chromatography, and structures were assigned using 0 MHz one- and two-dimensional 1H NMR spectroscopy. Pichia Man8GlcNAc was the same isomer as found in Saccharomyces, which arises by removing the alpha 1,2-linked terminal mannose from the middle arm of the lipid-oligosaccharide Man9GlcNAc (Byrd, J. Seebio lacto n neotetraose , Tarentino, A. L.
, Maley, F.
