Data-Role-gm-Structure-f

New stationary phase for hydrophilic interaction chromatography to separate chito-oligosaccharides with degree of polymerization 2-6.Zhai X(1), Zhao H(2), Zhang M(2), Yang X(3), Sun J(2), She Y(4), Dong A(2), Engineering, Harbin Institute of Technology, NO2 West Dazhi Street, Nangang District, Harbin, Heilongjiang Province 10, PR China; Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 81, PR China.Engineering, Harbin Institute of Technology, NO2 West Dazhi Street, Nangang District, Harbin, Heilongjiang Province 10, PR China.Engineering, Harbin Institute of Technology, NO2 West Dazhi Street, Nangang District, Harbin, Heilongjiang Province 10, PR China; Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 81, PR China.

Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 81, PR Xiangfang District, Harbin, Heilongjiang Province 10, China.Engineering, Harbin Institute of Technology, NO2 West Dazhi Street, Nangang District, Harbin, Heilongjiang Province 10, PR China; Key Laboratory of Agro-product Quality and Safety, Institute of Quality Standard & Testing Technology for Agro-Product, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun South Street, Haidian District, Beijing 81, PR China. Fucosylated oligosaccharides -functionalized stationary phase based on silica for hydrophilic interaction liquid chromatography (HILIC) was developed and showed great HILIC characteristics on separation for chito‑oligosaccharides. 2'-fucosyllactose was synthesized by grafting 3‑aminophenylboronic acid group to silica, and it was characterized by scanning electron microscopy (SEM), Fourier transform infrared spectroscopy (FT-IR), elemental analysis and thermal gravimetric analysis (TGA). Nucleobases and nucleosides were used to evaluate the retention property and to investigate retention mechanism by the models designed for description of partitioning and surface adsorption through adjusting ratio of water in the mobile phase.

Parameters affecting chromatography behavior such as ionic strength, buffer pH and column temperature were also investigated. Results have indicated that the retention mechanism was a combination of partitioning and surface adsorption, and the hydrogen bond seemed to be the main force for the retention behavior. Finally, the new 3‑aminophenylboronic acid-functionalized based on silica stationary phase was applied to separate chito-oligosaccharide samples with optimized mobile phase conditions and showed acceptable chromatograms.Fully automated two-dimensional high-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry for the determination of oligosaccharides in glycopeptides after enzymic fluorescence labeling.COE Program in the 21st Century, University of Shizuoka, 52-1 Yada, Suruga-ku, Two-dimensional high-performance liquid chromatography using an electrospray ionization time-of-flight mass spectrometry (2D-HPLC-ESI-TOF-MS) system was established for the on-line determination of asparagine-linked oligosaccharides in glycopeptides. The analysis of the oligosaccharides started with the enzymic transglycosylation reaction utilizing Endo-beta-N-acetylglucosaminidase resulting fluorescent oligosaccharides were specifically isolated from the non-fluorescent oligosaccharides with fluorescence detection after separation by the 1st dimension Amide- column. The fraction of fluorescent oligosaccharides was effectively trapped in the anion exchange column.

The trapped oligosaccharides were then separated by the 2nd dimension ODS column and sensitively determined by ESI-TOF-MS. Disialo-Asn (a model oligosaccharide) and several oligosaccharides liberated from ovalbumin could be efficiently separated by the 2D-HPLC and identified from the ESI-TOF-MS. Based on these results, the proposed 2D-HPLC-ESI-TOF-MS system may be useful for on-line oligosaccharide analyses. Although the analytical run time is still long, a high-throughput determination will be performed by optimization of the 2D-HPLC conditions.Observation of unique cross-linked lattices between multiantennary carbohydrates and soybean lectin. Presence of pseudo-2-fold axes of symmetry in complex type Biochemistry. 1994 May ;33(18)5614-22.