Mass-Values-Yields-Glycopeptides-Proportions-p

Electron microscopy revealed linear structures with weight average lengths of 2 nm (A) and 1 nm (B) corresponding to a massunit length of about 3 kDanm. The protein cores (17-19%) contain large amounts of threonine (over %), serine (17-24%), and proline (18-19%). Carbohydrate and sulfate account for approximately and%, respectively, and gas chromatography-mass spectrometry showed that the patterns of neutral and sialic acid-containing glycans are very similar in the two glycopeptides. Both contain a significant amount (7- mol %) of single GalNAc residues, the average oligosaccharide is about 4 sugar residues long, and the largest species observed are heptasaccharides. The major neutral and sialic acid-containing oligosaccharides are Fuc1-2Gal1-3GalNAcol and GlcNAc1-6(NeuGc2-Gal1-3)GalNAcol, respectively. Sialic acid is present as both N-acetyl- and N-glycoloyl-neuraminic acid.

Repeated extractions of the tissue with guanidinium chloride left approximately % of the mucus glycoproteins as an insoluble glycoprotein complex whereas exposure to dithiothreitol or high speed homogenization accomplished complete solubilization. The subunits obtained after reduction with dithiothreitol are larger than glycopeptides A and B, and fragments corresponding in size to the latter are obtained after cleavage with trypsin. Most of the mucins from rat small intestine thus occurs as an insoluble glycoprotein complex composed of subunits joined with disulfide bonds. The subunits contain two highly glycosylated regions with different lengths substituted with very similar oligosaccharides.Distribution of antigenic oligomannosyl side chains in the cell wall mannans of several strains of Candida tropicalis.In order to clarify the distribution of antigenic oligomannosyl side chains in the cell wall mannans of the pathogenic yeast Candida tropicalis, the chemical structure of mannans isolated from four C. tropicalis strains was investigated using nuclear magnetic resonance, two-dimensional homonuclear Hartmann-Hahn the distribution of oligomannosyl side chains in the mannans.

The linear side chain Manalpha1-3Manalpha1-(2Manalpha1-)(n)2Man [n or =2] is present in the mannans from C. tropicalis IFO589 and IFO 10, but not in the mannans from IFO199 and IFO 1647. The mannan of IFO589 is the only mannan with the branched side chains, Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)(n)2Man and Manalpha1-2Manalpha1-3[Manalpha1-6]Manalpha1-(2Manalpha1-)(n)2Man [n or =2]. However, 2'-fucosyllactose lacked the phosphate group and the beta-1,2-linked oligomannosyl side chain which are features of this group. The mannans of the C. tropicalis strains IFO589 and IFO 10 possessed the side chains containing an alpha-1,3-linked mannose residue previously observed in Candida albicans.Novel glucoamylase-resistant gluco-oligosaccharides with adjacent α-1, 6 branches at the non-reducing end discovered in Japanese rice wine, sake.

Honda C(1), Katsuta R(2), Yamada M(1), Kojima Y(1), Mamiya A(3), Okada N(3), Kawamura T(3), Totsuka A(4), Shindo H(1), Hosaka M(1), Nukada T(2), Tokuoka Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Tokyo, 156-82, Japan.Sakuragaoka, Setagaya-ku, Tokyo, 156-82, Japan.Agriculture, Tokyo University of Agriculture, 1-1-1 Sakuragaoka, Setagaya-ku, Sake, a traditional Japanese rice wine, contains various oligosaccharides (Sake oligosaccharides; SAOs) derived from rice starch. We previously found that SAOs reach a high degree of polymerization (DP). In 2'-Fucose lactose , we developed a hydrophilic interaction liquid chromatography-time-of-flightmass spectrometry SAOs with DP = 6, 7, and 8, which were named DP6-1, DP7-1, DP8-1 and DP8-2, respectively, were purified from sake and their structures were determined by two-dimensional NMR spectroscopy. These were novel oligosaccharides containing two α-1, 6 bonded branches on an α-1, 4-linked glucose main chain. Interestingly, adjacent double α-1, 6 branches that have not been identified in starch, were found in DP6-1, DP7-1, and DP8-1, suggesting the presence of the branching pattern in starch.

DP6-1 was poorly digested by fungal glucoamylase, and this may be attributed to its adjacent double branches at the non-reducing On the mechanism of ion transport through lipid membranes mediated by PEGylated cyclic oligosaccharides (CyPLOS) an ESR study.The mechanism underlying the ionophoric activity of CyPLOS (cyclic phosphate-linked oligosaccharide, 2), a carbohydrate-based synthetic ion transporter decorated with four tetraethylene glycol (TEG) chains, has been investigated by an integrated electron spin resonance (ESR) approach.