Study-Internalization-Lysosomal-Oligosaccharides-Fibroblasts-h

Oligosaccharides of known specific activity bearing a single phosphate in monoester linkage were internalized with Kuptake of 3 X (-7) M, whereas oligosaccharides bearing two phosphates in monoester linkage were internalized with a Kuptake of 3 X (-8) M. Thus, phosphorylated high mannose-type oligosaccharides appear to be the minimal structure required for recognition and uptake by the fibroblast receptor. The finding that the Kuptake for monophosphorylated oligosaccharides is 0-fold less than the reported Ki for mannose-6-phosphate indicates that the fibroblast phosphomannosyl receptor contains a binding site that recognizes features of the oligosaccharide in Design and synthesis of a click high-mannose oligosaccharide mimic emulating Cendret V(1), François-Heude M, Méndez-Ardoy A, Moreau V, Fernández JM, Université de Picardie Jules Verne, 33 Rue Saint-Leu, 039 Amiens Cedex 1, A dendritic click mannooligomer mimicking the high-mannose oligosaccharide Man(8) has been designed by replacing some of the inner mannopyranosyl subunits with triazole moieties; evaluation of its binding affinity towards the mannose-specific lectin concanavalin A revealed striking similarities between the click mimic and the natural Man(8).Synthesis and conformational analysis of arabinofuranosides, galactofuranosides Many microorganisms produce biologically important polysaccharides containing galactofuranosyl, arabinofuranosyl andor fructofuranosyl residues. Recent interest in identifying antibiotics that act by inhibiting the biosynthesis of these glycans has resulted in the development of new and efficient methods for the assembly of oligosaccharides containing furanose residues. In general, it is now possible to synthesize any furanose-containing oligosaccharide with reasonable efficiency.

In conjunction with these synthetic investigations, an increasing number of studies have probed the conformation of furanose rings, and a solid appreciation of the conformational preferences of key furanoside ring Isolation and characterization of novel phosphate-containing sialyloligosaccharides from normal human urine.Three phosphate-containing sialyloligosaccharides were isolated from normal human urine using charcoal adsorption, gel-filtration chromatography, ion-exchange chromatography and paper chromatography. Studies including gas-liquid chromatography of monosaccharide and disaccharide derivatives, methylation analysis, phosphate determination, ion-exchange chromatography and glycosidase and phosphatase treatments indicated the following three structures for the compounds isolated NeuAc(alpha 2-6)Gal(beta 1-4)GlcNAc(alpha)-P; NeuAc(alpha 2-3)Gal(beta 1-4)GlcNAc(alpha)-P; NeuAc(alpha 2-3)Gal(beta 1-3)GalNAc(alpha)-P. These sialyloligosaccharide 1-phosphates represent a novel class of oligosaccharides. Fucosylated Lactose are identical with the common sialyloligosaccharide end groups of glycoproteins and glycolipids. The excretion of these compounds in normal human urine may indicate the existence of a novel, as yet unrevealed pathway in the metabolism of complex carbohydrates.DOI 111j432-33984.

t81.xAdsorption and kinetic behavior of recombinant multifunctional xylanase in hydrolysis of pineapple stem and bagasse and their hemicellulose for A recombinant multifunctional xylanase (RMFXase) was utilized to hydrolyze xylan in pineapple stems and bagasse. The feasibility of using RMFXase and the two agricultural by-products for producing Xylo-oligosaccharides was demonstrated. Adsorption dynamics showed that lignin and cellulose delayed the adsorption equilibrium. The adsorption data fit the Langmuir-type adsorption isotherm. The extent of hydrolysis was quantified by measuring the reducing and total sugars in substrate-RMFXase complexes at °C. The substrates had similar hydrolysis characteristics.

Differences in the maximum hydrolysis rate (HR) and the time of maximum were obtained. The HR(max) of water-insoluble oat spelt xylan reached 556% after 2h. Analysis of the end-products using high-performance liquid chromatography indicated xylobiose and xylotriose were the major hydrolysis products, and the final degree of polymerization was greater than two. human milk oligosaccharides of the substrates and RMFXase are the key factors that determine the processing parameters of Xylo-oligosaccharide production.Identification of neutral and sialyl N-linked oligosaccharide structures from human serum glycoproteins using three kinds of high-performance liquid The distribution of neutral and sialyl N-linked oligosaccharides from human serum glycoproteins has been studied using three kinds of HPLC columns as described below.