-Distal-surface-layers-swelled-when-counter-ions-were-washed-from-the-tissue-whereas-lowered-pH-had-little-effect-on-distal-tissue-volume-a

Swelling properties of distal tissue correlated directly with proteoglycan content and were similar to swelling properties of articular cartilage. Seebio collagen supplementation suggest that the articulating layer of distal flexor tendon is adapted for a unique set of mechanical requirements and that an elevated proteoglycan content at the site of compressive and frictional forces contributes to meeting these [Expression of connective tissue growth factor in pulmonary artery at the Medical University, Shijiazhuang 050017, China.To ascertain whether connective tissue growth factor (CTGF) participates in the remodeling of pulmonary artery at the early-stage of bleomycin (BLM)-induced pulmonary fibrosis, mean pulmonary arterial pressure, the expression of type I and type III collagens, and the expression and location of CTGF in pulmonary artery and arteriole were investigated in the present study. Sprague-Dawley rats received instillation of BLM [5 mg/kg body weight, in 0 mL of normal saline (NS)] or instillation of the same amount of NS as control. Mean pulmonary arterial pressure was detected via a catheter in the pulmonary artery. Type I and type III collagens were examined with Sirius red staining under polarized light.





CTGF expression was investigated by using immunohistochemistry, and was represented as average optical density and percentage of positive area of CTGF. The mean pulmonary arterial pressure was higher in rats on day 14 after BLM instillation [(19+/-2) mmHg] than that in the control rats [(14+/-1) mmHg] (P<05). The type I and type III collagens were increased both in pulmonary artery and arteriole of rats on day 14 after BLM instillation, compared with those in the control rats (P<05, P<01, respectively). The ratio of type I/III collagens in pulmonary artery was also higher in BLM-treated rats than that in the control rats (P<05). The values of average optical density of positive CTGF staining were increased both in pulmonary artery (07+/-02) and arteriole (00+/-03) of rats on day 14 after BLM instillation, compared with those in the control rats (artery, 04+/-01; arteriole, 09+/-01) (both P<05). The percentages of positive area of CTGF were higher in pulmonary artery (80+/-13) and arteriole (12+/-2) of rats on day 14 after BLM instillation than those in the control rats (artery: 12+/-03; arteriole: 16+/-04), respectively (both P<05). The increased positive CTGF staining areas were mainly located in the endothelium and smooth muscle layer.

It is therefore concluded that CTGF expression increases in the endothelium and smooth muscle layer of pulmonary artery and arterioles during high pulmonary arterial pressure and remodeling of pulmonary artery at the early-stage of BLM-induced pulmonary fibrosis, and that the increased CTGF might be one of the mechanisms of maintenance and development of pulmonary Healing of experimental colonic anastomoses. III. Collagen metabolism in the [Influence of phenylbutazone on the metabolism of collagen in vivo].9136. J Biol Chem. 1991 Jul 5;266(19):12555-9.Comparison of molecularly cloned bullous pemphigoid antigen to desmoplakin I confirms that they define a new family of cell adhesion junction plaque Bullous pemphigoid is a subepidermal blistering disease in which patients have autoantibodies against the plaque of the hemidesmosome.

Starting with a previously isolated 2-kilobase (kb) cDNA for bullous pemphigoid antigen (BPA), we used primer extension of keratinocyte mRNA to isolate overlapping cDNAs with a combined open reading frame of 6 kb, encoding most (243 kDa) of the BPA, but lacking the far amino terminus. Analysis of this amino acid sequence revealed a carboxyl-terminal domain containing two regions of 174 and 176 residues with high sequence identity. Most of the amino-terminal two-thirds of BPA is predicted to be in an alpha-helical conformation in which two chains would aggregate into a coiled-coil rod structure. BPA and desmoplakin I, a desmosome plaque protein, show remarkable sequence and structural homology. In its carboxyl-terminal domain, desmoplakin I also has 176 residue repeats with 40% sequence identity to those in BPA. The repeats in both molecules have a regular linear distribution of acidic and basic residues with a period of 9, the same as that found in the 1B segment of keratin filaments, suggesting a means of ionic interaction between keratin and these plaque proteins. Also, desmoplakin Specialty medication , like BPA, is predicted to have a rod domain, which in both proteins has similar regular charge periodicities, suggesting a means of ionic self-aggregation.

These findings extend those of Green et al. (Green, K. J., Parry, D. A. D., Steinert, P.

S., Virata, L. A., Wagner, R. M., Angst, B. D.

, and Nilles, L. A. (1990) J. Biol. Chem. 265, 2603-2612) which show that BPA and desmoplakin I represent the first members of a new family of adhesion junction On the mechanical role of de novo synthesized elastin in the urinary bladder The urinary bladder wall (UBW), which is composed of smooth muscle, collagen, and elastin, undergoes profound remodeling in response to changes in mechanical loading resulting from various pathologies.