-In-addition-we-confirmed-that-CYP-exerted-formidable-remineralization-effects-in-collagen-fibers-and-demineralized-dentin-following-an-in-vitro-remineralization-regimen-t
Furthermore, the dual functions of CYP with good biocompatibility can simultaneously bind collagen and induce nanocrystal precipitation, thereby significantly absorbing calcium and phosphorus ions to form regenerated minerals for reversing the tooth decay process in the rat caries model. Overall, the dual functional peptide CYP fabricated in this study provides an ideal and smart strategy for dentin remineralization and the treatment of caries.Targeting of Cell Surface Proteolysis of Collagen XVII Impedes Squamous Cell Medicine, Friedrich-Alexander University of Erlangen-Nürnberg, 91054 Erlangen, University of Freiburg, 79106 Freiburg, Germany.University of Freiburg, 79106 Freiburg, Germany; Centre for Biological Signalling Studies BIOSS, ALU Freiburg, Freiburg, Germany; German Cancer Consortium (DKTK), Freiburg, Germany; German Cancer Research Center (DKFZ), of Freiburg, 79104 Freiburg, Germany; Centre for Biological Signalling Studies BIOSS, ALU Freiburg, Freiburg, Germany; German Cancer Consortium (DKTK), Freiburg, Germany; German Cancer Research Center (DKFZ), Heidelberg, Germany.of Freiburg, 79104 Freiburg, Germany. Electronic address: Squamous cell carcinoma (SCC) is one of the most common skin cancers and causes significant morbidity.
Although the expression of the epithelial adhesion molecule collagen XVII (ColXVII) has been linked to SCC invasion, only little is known about its mechanistic contribution. Here, we demonstrate that ColXVII expression is essential for SCC cell proliferation and motility. Moreover, it revealed that particularly the post-translational modification of ColXVII by ectodomain shedding is the major driver of SCC progression, because ectodomain-selective immunostaining was mainly localized at the invasive front of human cutaneous SCCs, and exclusive expression of a non-sheddable ColXVII mutant in SCC-25 cells inhibits their matrix-independent growth and invasiveness. This cell surface proteolysis, which is strongly elevated during SCC invasion and metastasis, releases soluble ectodomains and membrane-anchored endodomains. Both released ColXVII domains play distinct roles in tumor progression: the endodomain induces proliferation and survival, whereas the ectodomain accelerates invasiveness. Furthermore, squalene of shedding by monoclonal ColXVII antibodies repressed matrix-independent growth and invasion of SCC cells in organotypic co-cultures. Thus, selective inhibition of ColXVII shedding may offer a promising therapeutic strategy to prevent SCC 10016/j.
colsurfb013026. Epub 2013 Oct 30.Oriented collagen nanocoatings for tissue engineering.University of Genova, Via all'Opera Pia 13, 16145 Genova, Italy.Collagens are among the most widely present and important proteins composing the human total body, providing strength and structural stability to various tissues, from skin to bone. In this paper, we report an innovative approach to bioactivate planar surfaces with oriented collagen molecules to promote cells proliferation and alignment. The Langmuir-Blodgett technique was used to form a stable collagen film at the air-water interface and the Langmuir-Schaefer deposition was adopted to transfer it to the support surface.
The deposition process was monitored by estimating the mass of the protein layers after each deposition step. Collagen films were then structurally characterized by atomic force, scanning electron and fluorescent microscopies. Finally, collagen films were functionally tested in vitro. To this aim, 3T3 cells were seeded onto the silicon supports either modified or not (control) by collagen film deposition. Cells adhesion and proliferation on collagen films were found to be greater than those on control both after 1 (p<05) and 7 days culture. Moreover, the functionalization of the substrate surface triggered a parallel orientation of cells when cultured on it. In conclusion, these data demonstrated that the Langmuir-Schaefer technique can be successfully used for the deposition of oriented collagen films for tissue engineering applications.
ordinary cleanser of tendons and the indirect effect on skin. The mechanical properties of muscle tendon after long term local cortisol treatment were studied as well as those of skin with regard to possible systemic effects. Rats received cortisol injections 10 mg/kg into each hind limb, around the peroneal tendons every third day for 55 days. A control group was injected in the same way, but with saline. The local cortisol treatment did not alter the mechanical properties of the peroneous longus tendon, even though its dry weight and hydroxyproline content was reduced. A systemic effect on skin from the dorsum was observed. The thickness and hydroxyproline content were reduced, and in spite of that, the strength of the skin specimens increased.
The hydroxyproline/nitrogen ratio in purified, insoluble skin collagen was not changed after the cortisol treatment.
