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In the uremia group, they were submitted to 5/6 nephrectomy of the left kidney, total nephrectomy of the right kidney and median laparotomy, colotomy and colorraphy. Were collected for serum urea, creatinine and CRP dosages and the colonic segments were studied for evaluation of granulation tissue, collagen maturation, microvascular and myofibroblasts density, and cell viability. Through histochemical processing, microvascular density was evaluated by anti-CD34 monoclonal antibody marking, cell viability by cell proliferation nuclear antigen screening and myofibroblasts density with monoclonal anti-α-actin antibody. Computerized histometry was used for evaluations of collagens type I and III by the coloration of picrosirius.RESULTS: The group submitted to nephrectomy 5/6, compared to the sham group, show urea increase (p<0000) and higher C reactive protein (p=0142). Decrease of granulation tissue formation (border reepithelialization p=0,0196, angiofibroblast proliferation p=0379), mean collagen I (p=0,0009) and collagen III (p=0,016), microvascular density (p=0,0074), cell proliferation nuclear antigen (p<0,0000) and myofibroblasts (p<0,0001).





CONCLUSION: The uremia induced by nephrectomy 5/6 model establishes negative RACIONAL: A doença renal crônica atinge mais de 500 milhões de pessoas em todo o mundo. Neste contexto, as toxinas urêmicas estão relacionadas ao comprometimento OBJETIVO: Avaliar, na cicatrização de anastomoses colônicas de ratos urêmicos indicadores séricos e anatomopatológicos que possam estar relacionados com alteração do processo de reparação tissular.No grupo simulação eles foram submetidos à simulação da nefrectomia 5/6 do rim esquerdo, simulação de nefrectomia total do rim direito, laparotomia mediana, colotomia e colorrafia. No grupo uremia, eles foram submetidos à nefrectomia 5/6 do rim esquerdo, nefrectomia total do rim direito, laparotomia mediana, colotomia e colorrafia. Coletaram-se amostras de sangue para dosagens séricas da ureia, creatinina e proteína C reativa, e do cólon para processamentos histológicos e histoquímicos na avaliação do tecido de granulação, maturação de colágeno, densidade microvascular e de miofibroblastos, viabilidade celular cicatricial. Empregou-se a histometria computadorizada para as avaliações de colágenos tipos I e III, densidade microvascular pela marcação com anticorpo monoclonal anti-CD34, viabilidade celular pela pesquisa do antígeno nuclear de proliferação celular e a densidade de miofibroblastos com anticorpo monoclonal RESULTADOS: O grupo submetido à nefrectomia 5/6, em comparação ao grupo simulação, demonstraram aumentos da ureia sérica (p<0,0000) e proteína C reativa (p=0,0142), redução da formação de tecido de granulação (reepitelização de bordas p=0,0196, proliferação angiofibroblástica p=0,0379), porcentagens de colágeno I (p=0,0009) e colágeno III (p=0,016), densidade microvascular (p=0,0074) e miofibroblastos (p<0,0001) e antígeno nuclear de proliferação CONCLUSÃO: A uremia induzida pelo modelo de nefrectomia 5/6 determina impacto negativo no processo de cicatrização colônico.Conflict of interest statement: Conflict of interest: noneIn-situ preparation of silver salts/collagen fiber hybrid composites and their photocatalytic and antibacterial activities.

Ministry of Education, Hunan Provincal Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Ministry of Education, Hunan Provincal Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, Ministry of Education, Hunan Provincal Key Laboratory of Controllable Preparation and Functional Application of Fine Polymers, School of Chemistry and Chemical Engineering, Hunan University of Science and Technology, Xiangtan, To promote the utilization of collagen fiber, silver salts/collagen fiber hybrid composites with photocatalytic and antibacterial activities were successfully prepared in this study via the in-situ organic-inorganic process. powdered collagen , chemical composition and structure were discussed. Scanning electron microscopy (SEM) observation showed that the silver salts/collagen fiber hybrid composites were successfully prepared with silver salt particles (300-500 nm) distributing evenly on the surface of collagen fiber. collagen supplements -ray diffraction (XRD) patterns and Fourier transform infrared spectroscopy (FTIR) analysis provided strong evidence for the successful coating of silver salts on the surface of collagen fiber and the hybrid mechanism was subsequently discussed. The photocatalytic activity was evaluated by degrading methyl orange (MO) under ultraviolet (UV) light and visible light, respectively. The results indicated that AgCl/Collagen Fiber showed the most efficient photocatalytic activity under UV and visible light irradiation. Furthermore, the introduction of Ag+ endowed the photocatalysts with antibacterial performance, which was investigated by measuring the width of the bacteriostatic belts.