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gov Strong serological responses and HIV RNA increase following AS03-adjuvanted pandemic immunization in HIV-infected patients.L, Hirschel B, Siegrist CA; H1N1 Study Group.Collaborators: Siegrist CA, Posfay-Barbe K, Meier S, Bel M, Grillet S, Sealy G, Demeules J, Charvat S, Verdon M, Combescure C, Hirschel B, Calmy A, Nguyen A, Delhumeau-Cartier C, Gabay C, Guerne PA, Seebach J, Ribi C, Villard J, Dietrich PY, George AC, Favet L, van Delden C, Morard I, Mentha G, Giostra E, Hadaya K, Martin PY, Soccal P, Berney T, Noble S, Mohty B, Nagy M, Chalandon Y, Roosnek E, Passweg J, Kaiser L, Yerly S, Thomas Y, Wunderli W.INTRODUCTION: We aimed to determine the antibody responses and effect on viral load of the AS03-adjuvanted pandemic H1N1 vaccine in HIV-infected patients.METHOD: A total of 121 HIV-infected patients and 138 healthy subjects were enrolled in a prospective, open-label study. Healthy subjects received one dose and HIV-infected patients two doses of the AS03-adjuvanted split influenza an interval of 3-4 weeks.

The study was extended in 2010/2011 for 66 patients. Geometric mean titres (GMTs), seroprotection rates (post-vaccination titre ≥ 1:40) and HIV-1 RNA levels were measured before and 4 weeks after immunization.RESULTS: After two immunizations, the seroprotection rate (94 vs. 87%, respectively) and GMT (376 vs. 340, respectively) in HIV-infected patients were as high as in healthy subjects after one dose, regardless of CD4 cell count. Four weeks after immunization, HIV RNA was detected in plasma samples from 40 of 68 (58%) previously aviraemic patients [median 152 HIV-1 RNA copies/mL; interquartile range (IQR) 87-509 copies/mL]. Subsequent measures indicated that HIV RNA levels had again declined to <20 copies/mL in most patients (27 of 34; 79%).

Following (nonadjuvanted) influenza immunization in 2010/2011, HIV RNA levels only slightly increased (median final level 28 copies/mL) in three of 66 (4%) previously aviraemic patients, including two of 25 (8%) patients in whom an increase had been elicited by AS03-adjuvanted vaccine the year before.CONCLUSION: Most HIV-infected patients developed seroprotection after two doses of AS03-adjuvanted pandemic vaccine. A transient effect on HIV RNA levels was observed in previously aviraemic patients. A booster dose of the nonadjuvanted influenza vaccine containing the A/09/H1N1 strain the following year did not reproduce this finding, indicating a non-antigen-specific adjuvant effect.3296. Ugeskr Laeger. 2013 May 13;175(20):1447.

[ Get it now of Pandemrix swine flu vaccine containing squalene]. Ugeskr Laeger. 2013 May 13;175(20):1444.Extracellular matrix collagen alters cell proliferation and cell cycle progression of human uterine leiomyoma smooth muscle cells.Uterine leiomyomas (ULs) are benign tumors occurring in the majority of reproductive aged women. Despite the high prevalence of these tumors, little is known about their etiology. A hallmark of ULs is the excessive deposition of extracellular matrix (ECM), primarily collagens.

Collagens are known to modulate cell behavior and function singularly or through interactions with integrins and growth factor-mediated mitogenic pathways. To better understand the pathogenesis of ULs and the role of ECM collagens in their growth, we investigated the interaction of leiomyoma smooth muscle cells (LSMCs) with two different forms of collagen, non-polymerized collagen (monomeric) and polymerized collagen (fibrillar), in the absence or presence of platelet-derived growth factor (PDGF), an abundant growth factor in ULs. Primary cultures of human LSMCS from symptomatic patients were grown on these two different collagen matrices and their morphology, cytoskeletal organization, cellular proliferation, and signaling pathways were evaluated. Specialty medication showed that LSMCs had distinct morphologies on the different collagen matrices and their basal as well as PDGF-stimulated proliferation varied on these matrices. These differences in proliferation were accompanied by changes in cell cycle progression and p21, an inhibitory cell cycle protein. In addition we found alterations in the phosphorylation of focal adhesion kinase, cytoskeletal reorganization, and activation of the mitogen activated protein kinase (MAPK) signaling pathway. In conclusion, our results demonstrate a direct effect of ECM on the proliferation of LSMCs through interplay between the collagen matrix and the PDGF-stimulated MAPK pathway.

In addition, these findings will pave the way for identifying novel therapeutic approaches for ULs that target ECM proteins and their Conflict of interest statement: Competing Interests: We have the following interest: Arash Mahdian is employed by Wolfram Research. There are no patents, products in development or marketed products to declare. This does not alter our adherence to all the PLOS ONE policies on sharing data and materials, as detailed online in the guide for authors.10016/j.atherosclerosis010422. Epub 2010 Apr 22.Evaluation of methods for the determination of cholesterol absorption and Cardiovascular disease risk and its associated complications correlate positively with circulating cholesterol levels.





Plasma cholesterol levels are maintained by reciprocally related endogenous cholesterol synthesis and cholesterol absorption from dietary and biliary sources.