Changes-Day-Feed-l

A diurnal variation in milk lactose was seen, which was the inverse of the oligosaccharides. Significant negative correlations between lactose and oligosaccharides were seen in the afternoon and evening (p less than5). The minimal mean value of lactose at 190 h is 584 +- 58 gl and the maximal mean values of oligosaccharides was 173 +- 28 gl. The data are discussed with regard to other milk constituents and to their possible physiological relevance.Nano-HPLC-mass spectrometry and MEKC for the analysis of oligosaccharides from The separation of oligosaccharides derivatized with various esters of aminobenzoic acid by means of reversed-phase nano-HPLC (nHPLC) with on-line ESI mass spectrometry and off-line MALDI-TOF mass spectrometry as well as MEKC is described. For lacto-n-neotetraose , ethyl and butyl aminobenzoates and heptyloxyaniline were used as derivatization agents for homologous maltodextrins and oligosaccharides from human milk.

Four different C(18) stationary phases were tested for this purpose because the type of stationary phase was shown to have a dramatic effect on the performance of the separation. Optimal results were obtained using n-butyl aminobenzoate as label and an encapsulated ODS stationary phase. The on-line coupling of nHPLC to ESI MS allowed to separate and identify various oligosaccharides from human milk. This technique enabled the exact attribution of the molecular structure to a signal in the chromatogram. In a second approach oligosaccharides were separated by nHPLC and subsequently fractionated. The fractions were analyzed by MALDI-TOF mass spectrometry. The results obtained by this approach confirmed the ESI MS data.

An analogous separation profile was obtained by using sodium dodecyl sulfate in MEKC, which proves that the retention mechanisms of both techniques are Three novel oligosaccharides with the sialyl-Lea structure in human milk isolation by immunoaffinity chromatography.Kitagawa H(1), Nakada H, Kurosaka A, Hiraiwa N, Numata Y, Fukui S, Funakoshi I, We have determined the structures of three novel oligosaccharides isolated from human milk using the monoclonal antibody MSW 113. These oligosaccharides were purified by affinity chromatography on a column of the immobilized monoclonal antibody and by high-performance liquid chromatography. From the results of 0-MHz 1H NMR spectroscopy and fast atom bombardment-mass spectrometry, their structures were deduced to be (formula; see text) These oligosaccharides bound to MSW 113 to nearly the same extent as sialyl-Lea hexasaccharide but bound to Comparative Analysis of Free and Glycoconjugates Oligosaccharide Content in Milk Zhong P(1), Yang Y(1), Han T(1), Huang W(1), Liu Y(1), Gong G(1), Huang L(1), Lu Human milk is important for infant growth, and oligosaccharides are one of its main functional nutrients. To enable a systematic comparison of free oligosaccharide and glycoconjugate content in milk from different species, the phenol-sulfuric acid and resorcinol assays were combined to determine the content. Using Oligosaccharides , the method revealed that human milk contained the highest amount of total, neutral (94 ±1 gL), and sialylated (31 ±1 gL) free oligosaccharides, followed by goat milk, with neutral 35 ±15 gL) and sialylated 92 ±16 gL) free oligosaccharides and at a distance by bovine and yak milk. The highest total glycoconjugate content was detected in yak milk 98 ±11 gL), followed by human, bovine, and goat milk.

These findings suggest that goat milk is the best source of free oligosaccharides in infant formula and functional dairy products and yak milk is the best source of Synthesis of the human milk oligosaccharide lacto-N-tetraose in metabolically Human milk oligosaccharides (HMOs) constitute the third most abundant solid component of human milk. HMOs have been demonstrated to show positive effects on the infant's well-being. Despite numerous studies, more physiological analyses of single compounds are needed to fully elucidate these effects. Although being one of the most abundant core structures in human milk, the HMO lacto-N-tetraose construction of the first E. coli strain capable of producing LNT in vivo. The strain was constructed by chromosomally integrating the genes lgtA and wbgO, encoding β-1,3-N-acetylglucosaminyltransferase and β-1,3-galactosyltransferase. In shake-flask cultivations, the strain yielded a total concentration of 219±3 mg L(-1) LNT (LNT in culture broth and the cell pellet).