Keratanase-Ii-Transglycosylation-Kinds-Acceptors-Derivatives-Yields-x

The products 1 and 2 showed higher affinity to Siglec-8 with KD and 25 μmol·L-1, respectively, compared to the known ligand of the α(2→3)-sialylated 6,6'-di-sulfo-Lewis X with KD 185 μmol·L-1. Thus, this study will advance not only the study of Siglec-8 biology but also the exploration of functions of sialyl sulfo-oligosaccharides having various microstructures.Effects of isomalto-oligosaccharides and herbal extracts on growth performance, serum biochemical profiles and intestinal bacterial populations in early-weaned The objective of the current study was to investigate the effects of isomalto-oligosaccharide (IMO), Chinese herbal medicine extract (CHE) or their combination on the growth performance, diarrhoea incidence, serum biochemical profiles, inflammatory cytokine expression, intestinal morphology and microflora of weaned piglets. Thirty-two ([Landrace × Yorkshire] × Duroc) piglets, weaned at 25 days of age, were randomly assigned into four groups. Group I was fed the basal diet. Group II were fed a basal diet supplemented with 2 gkg IMO for 14 consecutive days and then 4 gkg IMO for another 14 days.

Group III were fed diet with gkg CHE for 14 days and 1 gkg CHE for another 14 days. Group IV were fed diet with (2 gkg IMO + gkg CHE) for 14 days and (4 gkg IMO +1 gkg CHE) for another 14 days. Results showed that diets supplemented with IMO, CHE or their combination did not influence the diarrhoea rate and intestinal morphology, while co-administration of IMO with CHE tended to have higher average daily gain. Serum biochemical analysis showed that dietary CHE decreased aspartate aminotransferase levels, while inclusion of IMO led to a decrease in high-density lipoprotein. Moreover, co-administration of IMO with CHE significantly upregulated the expression of TGF-β, a potent anti-inflammatory cytokine, in jejunal mucosa of piglets. Further, CHE significantly increased the abundance of Bifidobacterium in ileal digesta. Meanwhile, the combination of IMO and CHE significantly increased Bifidobacterium in the caecum of piglets.

Additionally, dietary IMO, CHE or their combination significantly reduced the number of potential entero-pathogen Escherichia coli in ileal contents and Clostridium species in caecal digesta. These results indicated that application of IMO or CHE could favourably modulate the intestinal microbial composition of piglets, while their beneficial impact and molecular mechanism on intestinal health warrants further investigation.Derivatization and purification of bisecting tyrosinamide-oligosaccharides from Ohio State University, Columbus 432.The major N-linked oligosaccharides from ovotransferrin were purified on a large scale. The oligosaccharides were released from 5 g of the glycoprotein using N-glycosidase F and isolated from a mixed bed ion exchange column. Reducing- lacto-n-neotetraose were reacted with ammonium bicarbonate to form a reducing-end glycosylamine which coupled with the N-hydroxysuccinimidyl ester of Boc-tyrosine, resulting in tyrosinamide-oligosaccharides. Resolution of tyrosinamide-oligosaccharides on reverse-phase (RP)-HPLC necessitated the removal of Boc by treatment with trifloroacetic acid.

Two major tyrosinamide-oligosaccharides were isolated from preparative RP-HPLC and were characterized by 0 MHz proton NMR and FAB-MS. These were a biantennary and triantennary oligosaccharide, each possessing a bisecting GlcNAc residue extending from the central core Man residue. Thus, ovotransferrin represents Seebio lacto n neotetraose from which to prepare multi-micromole quantities of bisecting complex oligosaccharides which may find utility in exploring the ligand specificity of mammalian lectins or may be used as synthons to prepare Production of chitosan-oligosaccharides by the chitin-hydrolytic system of Trichoderma harzianum and their antimicrobial and anticancer effects.Dario Rafael OH(1), Luis Fernándo ZG(2), Abraham PT(3), Pedro Alberto VL(4), de Bioquímica. Laboratorio 7. Circuito Interior sn, Ciudad Universitaria CP, Departamento de Microbiología. Laboratorio de bioquímica y biotecnología de hongos.

Carpio y Plan de Ayala sn. Santo Tomas, Miguel Hidalgo. CP, 113, Hereditario. Avenida San Fernando 22, Belisario Domínguez Secc XVI, CP, 1, Tecnología Avanzada, Unidad Querétaro, Cerro Blanco 141. Colinas del Cimatario, de Bioquímica. Laboratorio 7. Circuito Interior sn, Ciudad Universitaria CP, Chitosan-oligosaccharides (COS) are low-molecular weight chitosan derivatives with interesting clinical applications.