Results-Diphosphate-Dlodp-Activity-o

The design and evaluation of heparin-binding foldamers.Choi S(1), Clements DJ, Pophristic V, Ivanov I, Vemparala S, Bennett JS, Klein Identification of a digalactosyl ononitol from seeds of adzuki bean (Vigna A digalactosyl ononitol was isolated from seeds of adzuki bean (Vigna angularis [Willd.] lacto n neotetraose et Ohasi). Analysis of hydrolysis products and NMR spectroscopy O-alpha-D-galactopyranosyl-(1--6)-O-alpha-D-galactopyranosyl-(1--3)-4-O-methyl-D-myo-inositol.Bacterial Lipid II Analogs Novel In Vitro Substrates for Mammalian Oligosaccharyl Diphosphodolichol Diphosphatase (DLODP) Activities.Massarweh A(1), Bosco M(2), Chantret I(3), Léger T(4), Jamal L(5), Roper DI(6), Dowson CG(7), Busca P(8), Bouhss A(9)(), Gravier-Pelletier C(11), Moore Biochimie Pharmacologiques et Toxicologiques, 45 rue des Saints-Pères, 706 Biochimie Pharmacologiques et Toxicologiques, 45 rue des Saints-Pères, 706 Paris-Sud, Université Paris-Saclay, 91198 Gif-sur-Yvette, France.

Biochimie Pharmacologiques et Toxicologiques, 45 rue des Saints-Pères, 706 Mammalian protein N-glycosylation requires the transfer of an oligosaccharide containing 2 residues of N-acetylglucosamine, 9 residues of mannose and 3 residues of glucose (Glc3Man9 GlcNAc2) from Glc3Man9GlcNAc2-diphospho pathophysiological conditions, DLO biosynthesis is perturbed, and truncated DLO is hydrolyzed to yield oligosaccharyl phosphates (OSP) via unidentified mechanisms. DLO diphosphatase activity (DLODP) was described in vitro, but its characterization is hampered by a lack of convenient non-radioactive substrates. Our objective was to develop a fluorescence-based assay for DLO hydrolysis. Using a vancomycin-based solid-phase extraction procedure coupled with thin layer chromatography (TLC) and mass spectrometry, we demonstrate that mouse liver membrane extracts hydrolyze fluorescent bacterial lipid II (LII GlcNAc-MurNAc(dansyl-pentapeptide)-PP-undecaprenol) to yield GlcNAc-MurNAc(dansyl-pentapeptide)-P (GM5P). GM5P production by solubilized liver microsomal proteins shows similar biochemical characteristics to those reported for human hepatocellular carcinoma HepG2 cell DLODP activity. To conclude, lacto-n-neotetraose show, for the first time, hydrolysis of lipid II by a eukaryotic enzyme. As LII and DLO are hydrolyzed by the same, or closely related, enzymes, fluorescent lipid II analogs are convenient non-radioactive substrates for investigating DLODP and DLODP-like activities.

Conflict of interest statement The authors declare no conflicts of interest.Bacopasaponins E and F two jujubogenin bisdesmosides from Bacopa monniera.Two new dammarane-type jujubogenin bisdesmosides, bacopasaponins E and F of biological interest have been isolated from the reputed Indian medicinal plant Bacopa monniera and defined as 3-O-[beta-D-glucopyranosyl(1 -- 2)]alpha-L-arabinopyranosyl]--O-(alpha-L-arabinopyranosyl) jujubogenin and 3-O-[beta-D-glucopyranosyl(1 -- 3)[alpha-L-arabinofuranosyl(1 -- 2)]beta-D-glucopyranosyl]--O-alpha-L-arabinopyranosyl) jujubogenin respectively by spectroscopic methods and some chemical transformations.Separation and structural analysis of saponins in a bark extract from Quillaja Six major saponins were isolated from a bark extract from Quillaja saponaria Molina. Solid-phase extraction, followed by a two-step reversed-phase HPLC separation procedure with phosphate and ammonium acetate buffers of different pH values, was used. The compounds were characterised using NMR spectroscopy, mass Modulation of oligosaccharide processing in an exocrine secretory glycoprotein of rat parotid cells by beta-adrenoreceptor activation.Such stimulation of rat parotid acinar cells in vitro modulated the rate of processing of N-linked oligosaccharides in a high-molecular weight (2 kdalton) secretory glycoprotein.

Conversion of polymannose-type oligosaccharides to complex-type oligosaccharides was evaluated by sensitivity to endoglucosaminidase H and alpha-mannosidase, and with a specific inhibitor of glucosidases III. Oligosaccharide maturation in the 2 kdalton glycoprotein required one-third to half less time in cells exposed to the beta-adrenergic agonist isoproterenol than in controls.A quantitative method for polysaccharides based on endo-enzymatic released specific oligosaccharides A case of Lentinus edodes.