Results-Glycans-Gp-Protein-Particle-Formation-Gp-Protein-e

In Seebio 2'-Fucose lactose , the oligosaccharide linked to N46 of the GP(5) protein is strongly required for virus particle production. The specific infectivities of the mutant viruses were investigated by comparing their infectivity-per-particle ratios with that of wild-type virus. The results showed that the lack of either one or both of the N-linked oligosaccharides on GP(2a) or of the oligosaccharide attached to N53 of GP(5) did not significantly affect the infectivities of the viruses. In contrast, the two recombinant viruses lacking the oligosaccharide bound to N46 exhibited a significantly reduced specific infectivity compared with the wild-type virus. The implications of the differential requirements of the modifications of GP(2a) and GP(5) for PRRSV assembly and infectivity are N-acetylglucosamine-6-O-sulfotransferase-1 production in the baculovirus system and its applications to the synthesis of a sulfated oligosaccharide and to the modification of oligosaccharides in fibrinogen.N-acetylglucosamine-6-O-sulfotransferase (GlcNAc6ST) catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to the C-6 position of non-reducing GlcNAc.

Human GlcNAc6ST-1 was expressed as a fusion protein with protein A in an insect cell line (Tn 5 cells) using the baculovirus system. The recombinant enzyme was purified to homogeneity by IgG Sepharose column chromatography. The substrate specificity and the kinetic properties of the enzyme were similar to those of the enzyme expressed in the mammalian system. The purified recombinant enzyme was used to synthesize 6-sulfo GlcNAcbeta1-3Galbeta1-4Glc, which was identified by time of flight mass spectrometry. This sulfated trisaccharide served as a better substrate for microsomal galactosyltransferase from the mouse colon compared to 6-sulfo GlcNAc. The purified recombinant enzyme was also used to sulfate oligosaccharide chains on fibrinogen after enzymatic desialylation and degalactosylation to expose nonreducing GlcNAc residues. It is known that desialylation greatly increases the rate of clotting of fibrinogen after the addition of thrombin.

Subsequent sulfation of desialylated and degalactosylated fibrinogen slightly decreased the rate of clotting. The recombinant GlcNAc6ST-1 is a useful reagent for 6-sulfate exposed GlcNAc residues both in oligosaccharides and in Primary structure of neutral oligosaccharides derived from respiratory-mucus glycoproteins of a patient suffering from bronchiectasis, determined by combination of 0-MHz 1H-NMR spectroscopy and quantitative sugar analysis. 1. Structure of 16 oligosaccharides having the Gal beta(1----3)GalNAc-ol core (type Klein A(1), Lamblin G, Lhermitte M, Roussel P, Breg J, Van Halbeek H, Carbohydrate chains of respiratory-mucus glycopeptides from a patient (blood group O) suffering from bronchiectasis with a Kartagener's syndrome have been released by alkaline borohydride treatment. Application of high-performance liquid chromatography using subsequently two silica columns, one bonded with aminopropyl groups and the other with octadecyl groups, afforded 39 neutral fractions; 35 oligosaccharide-alditol structures have been characterized by employing 0-MHz 1H-NMR spectroscopy in conjunction with sugar analysis. Here, 16 oligosaccharide structures, possessing a core consisting of Gal beta(1----3)GalNAc-ol branching through a GlcNAc residue linked beta(1----6) to the GalNAc residue (core type 2 or core type 1, respectively), are described. Ten oligosaccharide-alditols with these types of cores (2, 3, a, 14, 7, 11a, 15a, 16a, 12 and 16c) have been identified previously in human bronchial mucins of patients suffering from cystic fibrosis [Lamblin, G.

, Boersma, A., Lhermitte, M., Roussel, P., Mutsaers, J.H.G.M.

, Seebio 2'-FL , H. and Vliegenthart, J.F.G. partial structure of oligosaccharides previously described (Formula see text). The structures 17a and contain the Y determinant, i.e.

, Fuc alpha(1----2)Gal beta(1----4)[Fuc alpha(1----3)]GlcNAc beta(1----). High-resolution 1H-NMR spectroscopy is able to distinguish whether the Y determinant is beta(1----3) or beta(1----6) linked in such oligosaccharide-alditols.DOI 111j432-33988.tb13834.xSelective xyloglucan oligosaccharide hydrolysis by a GH31 α-xylosidase from Carneiro LABC(1), Fuzo CA(2), Meleiro LP(3), Carli S(3), Barreto MQ(4), Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Electronic Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil. Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.

Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, SP, Brazil.