Results-Proportion-Arabinosyl-Chains-Proportion-Oligosaccharide-Chains-d

GLC-MS analysis showed that, after methylation the short, neutral oligosaccharide fraction consisted of at least 22 kinds of di- to tetra-saccharide alditols, such as Gal-(1--4)-Rha-ol (a major component), Ara-(1--4)-Rha-ol, Glc-(1--4)-Rha-ol, Ara--Ara--Ara-ol, and Ara--Ara--Ara--Ara-ol (minor components) in addition to heteroglycosyl alditols. After deesterification, PG-2 and PG-3 were digested with endo-alpha-(1--4)-polygalacturonase again, and the enzyme-resistant intermediate size fraction (PG-2') was purified. Component sugar analysis indicated that PG-2' contained 2-Me-Fuc, 2-Me-Xyl, apiose (Api), aceric acid (AceA), 3-deoxy-D-lyxo-heptulosaric acid (Dha), and 3-deoxy-D-manno-2-octulosonic acid (Kdo) in addition to Rha, Fuc, Ara, Xyl, Man, Gal, Glc, GalA, and GlcA. Lithium degradation of PG-2' gave mainly a pentosyl--6-deoxyhexosyl--6-deoxyhexosyl--pentitol fragment, with some neutral di- and tri-saccharide alditols, including a pentosyl--deoxyhexitol. Methylation analysis of these degradation products indicated that they contained terminal Rha, Araf, Fuc, Xyl, and Gal, 4-linked Rha, 3-linked Fuc, 3-linked Ara, and 3'-linked Api. Bupleuran 2IIc was eluted as essentially a single peak on gel filtration on Sepharose CL-6B.

The neutral sugar content of the successive fractions increased with increasing molecular weight, but each fraction also Abnormal galactoside excretion in urine of a patient with early myoclonic An abnormal carbohydrate pattern was found in urine of a patient with early myoclonic epileptic encephalopathy. Three major oligosaccharides have been isolated from the urine; structural studies including sugar analyses, methylation procedure and enzymatic hydrolysis allow us to propose the following structures beta-Gal-(1 leads to 3)-Gal beta-Gal-(1 leads to 3)-beta-Gal-(1 leads to 3)-Glc beta-Gal-(1 leads to 3)-beta-Gal-(1 leads to 3)-Gal Such oligosaccharide structures have not previously been described in any biological fluid. The origin of these compounds, and the possibility of a specific and Department of Chemistry of Medicinal Natural Products, School of Pharmacy, The total synthesis of batatoside L (1), a resin glycoside possessing cytotoxicity against laryngeal carcinoma cells, has been completed in a highly convergent manner. 2'-Fucose lactose in this total synthesis was the efficient construction of the 18-membered macrolactone framework through the Corey-Nicolaou macrolactonization approach.Four acarviosin-containing oligosaccharides identified from Streptomyces coelicoflavus Z656 are potent inhibitors of alpha-amylase.Four aminooligosaccharides were isolated and purified from the culture filtrate of Streptomyces coelicoflavus Z656. Their chemical structures were determined by electrospray ionization tandem mass spectrometry (ESI-MSMS) and two-dimensional nuclear magnetic resonance (NMR) spectroscopy.

The names acarviostatins 3, I3, II3, and I3 were given to the oligomers due to their acarviosin core structures. Acarviostatins II3 and I3, which contain three and four acarviosin-glucose moieties, respectively, were identified as novel compounds. The four acarviostatins were all mixed noncompetitive inhibitors of porcine pancreatic alpha-amylase (PPA). The inhibition constants Acarviostatin II3 is the most effective alpha-amylase inhibitor known to date, with a K(i) value 2 times more potent than acarbose.A rapid and highly sensitive microchip electrophoresis of mono- and mucin-type oligosaccharides labeled with 7-amino-4-methylcoumarin.A selective separation method using a poly(methylmethacrylate) microchip was developed for 7-amino-4-methylcoumarin-labeled saccharides in a crude reaction mixture. In an alkaline borate buffer, saccharide derivatives formed strong anionic borate complexes.

These complexes moved from the cathode to the anode in an electric field and were detected near the anode. Excess labeling reagents and other foreign substances remained at the inlet reservoir. A confocal fluorimetric detection system enabled the determination of monosaccharide derivatives with good linearity between at least 5 and 0 nM, corresponding to  fmol to 1 pmol per injection. The lower limit of detection were comparable to reported values using fluorometric detection, capillary electrophoresis, or liquid chromatography. Application of the method showed excellent resolution in the analysis of O-linked glycans chemically released Synthesis of regioselectively sulfated xylodextrins and crystal structure of sodium methyl beta-D-xylopyranoside 4-O-sulfate hemihydrate.Methyl xylobioside and methyl xylotrioside were prepared from the peracetylated anomeric xylosyl trichloroacetimidates by reaction with methanol followed by Zemplén deacetylation.